A sensitive, safe and cost-effective high-throughput homogeneous assay of cell viability by fluorescence or absorbance measurement. Detection method/readout Microplate reader (absorbance), Microplate reader (fluorescence) Assay type/options Endpoint assay, High-throughput assay, Homogeneous assay, Real-time imaging Resazurin Cell Viability Assay What is the difference between MTT, XTT and resazurin? MTT and XTT are colorimetric based assays, while resazurin can be measured using colorimetric or fluorescence detection. MTT is not a soluble product, so the cells must be lysed to solubilize the formazan salt before absorbance can be measured. XTT and resazurin do not require cell lysis, allowing kinetic monitoring of the same samples at different timepoints. View more FAQs What is the difference between resazurin and alamarBlue®? AlamarBlue® contains resazurin and additional compounds to prevent the over-reduction of resazurin to a non-fluorescent product. These additives also slow the rate of generation of the fluorescent product. Consequently, the alamarBlue® assay requires longer incubation times compared to resazurin. View more FAQs Why does my sample with the most dense cells seem have the least amount of resazurin fluorescence? Resazurin is reduced by cells to the fluorescent product resorufin. Resorufin can be reduced further to a non-fluorescent compound. Therefore the densest wells may have lowest fluorescence due to over-reduction of the substrate. Please see the product information sheet for more details. The kit protocol provides general guidelines and may need to be optimized empirically for your experimental system. You may need to vary cell density or assay incubation time to ensure that your samples fall in the linear range of the kit. View more FAQs Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes. Size Catalog # price Shipping, Handling & Tax (CA only) not included in price. Learn more Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.Resazurin Cell Viability Assay offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. This assay has excellent performance compared to other resazurin-based cell proliferation kits such as alamarBlue®, PrestoBlue®, or CellTiter-Blue®.Single-step, homogeneous, high-throughput cell quantitationFor fluorescence- or absorbance-based measurementSensitive, requires as few as 80 cells for reproducible resultsWidely used in cultured mammalian cells, bacteria, protozoa, yeast, fungiCell lysis not required, allows long-term kinetic monitoringNon-toxic, non-radioactive, non-carcinogenic, simple disposalReady-to-use solutionResazurin detects cell metabolic by converting from a nonfluorescent dye to the highly red fluorescent dye resorufin in response to chemical reduction of growth medium resulting from cell growth. Continued cell growth maintains a reduced environment while inhibition of growth maintains an oxidized environment. Reduction related to growth causes the redox indicator to change from the oxidized form (nonfluorescent, blue) form to the reduced form (fluorescent, red).Reduced resazurin can be detected using fluorescence (Ex/Em 530-560/590 nm) or absorbance at 570 nm. The fluorescent or colorimetric signal is proportional to the number of living cells in the sample. Results can obtained between 1-24 hours after adding substrate. Resazurin solution is supplied ready-to-use in 25 mL (2500 assay) or 100 mL (10,000 assay) sizes.See our full selection of Cell Viability Apoptosis Assays.alamarBlue is a registered trademark of Trek Biosystems. CellTiter-Blue is a registered trademark of Promega Corporation. PrestoBlue is a registered trademark of Thermo Fisher Scientific. 1. Arterioscler, Thromb, Vasc Biol (2010) 30(10), 1940–1948. DOI:10.1161/ATVBAHA.110.2059972. J Clin Endocrinol Metab, October 2010, 95(10):E181–E191 doi: 10.1210/jc.2010-05813. Nucleic Acids Res (2010) 38(22), 8131–8140. doi:10.1093/nar/gkq6974. Scand J Clin Lab Invest. (2010) 70(7), 512-8. doi: 10.3109/00365513.2010.5212555. Tissue Eng A (2011) 17(1-2), 37-44. http://doi.org/10.1089/ten.tea.2010.01886. Biomaterials (2011) 32(1), 128-36. doi: 10.1016/j.biomaterials.2010.09.006.7. Food Chem (2012) 135(3), 1533-1538. https://doi.org/10.1016/j.foodchem.2012.06.0588. Drug Des Dev Therapy (2013) 7, 529–544. http://dx.doi.org/10.2147/DDDT.S451629. Langmuir (2013) 29(46), 14254-64. doi: 10.1021/la403533r10. PLoS ONE (2013) 8(10), e76264. doi:10.1371/journal.pone.007626411. ACS Nano (2015) 9(2), 1219-1235. https://doi.org/10.1021/nn504890z12. Antimicrob Agents Chemother (2016) 60(7), 4183–4196. doi:10.1128/AAC.03021-1513. Curr Prot Toxicol (2016) 68, 2.24.1-2.24.15. doi: 10.1002/cptx.114. J Biol Chem (2016) 291, 21869-21879. doi: 10.1074/jbc.M115.71216615. Curr Prot Stem Cell Biol (2018) 26(1), 5C.3.1-5C.3.19. DOI:10.1002/9780470151808.sc05c03s2616. Int J Biol Macromol (2018) 113(1), 132-141. https://doi.org/10.1016/j.ijbiomac.2018.02.069 A convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or fluorescence microscopy. Detailed View ATP-Glo Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay involves a single addition of ATP-Glo Detection Cocktail directly to cells in culture medium. Detailed View Quantify live cell numbers based on their endogenous esterase activity and plasma membrane integrity using green fluorescence. For microplate reader, microscopy, or flow cytometry. Detailed View These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugate for detecting caspase-3 activation and phosphatidylserine (PS) translocation. Detailed View Resazurin (also known as alamarBlue ) is the N-oxide of the fluorescent dye resorufin and is useful for detecting reductive activities in cells. It has been widely used for measuring cell proliferation (1,2) and mitochondrial metabolic activity. Resazurin itself is non-fluorescent until it is reduced to the highly red fluorescent resorufin (λEx/λEm: 563/587 nm). Detailed View A convenient assay for staining live (green) and dead (red) cells within the same cell population by flow cytometry or fluorescence microscopy. Detailed View Biotium implements a Quality System, certified by QAS according to Standard QAS ISO 9001:2015. Certificate No. US3564